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| Status |
Public on Nov 22, 2024 |
| Title |
Expression data from Methionine normal or deprived culture glioblastoma cell line U251 |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Recent research highlights the significant impact of methionine metabolism on glioma progression. An increasing amount of compelling evidence bridges long non-coding RNAs to abnormal metabolism in gliomas. However, the specific role of long non-coding RNAs in methionine metabolism regulating glioma progression remains unclear. This study reveals that methionine deprivation inhibits the proliferation, migration, and invasion capabilities of gliomas. Interestingly, the expression of TP53TG1, a long non-coding RNA, is also suppressed. TP53TG1 is highly expressed in gliomas and associated with poor patient outcomes. Subsequently, our data proves that inhibition of TP53TG1 suppresses glioma cell proliferation and the epithelial-mesenchymal transition process both in vitro and in vivo. Ultimately, we found that the underlying mechanism involves a competing endogenous RNA regulating network, in which TP53TG1 modulates the target protein STK17B by competitively binding to miR-96-5p, thus regulating glioma progression. These findings suggest that targeting methionine deprivation could be a promising approach for the clinical treatment of glioma.
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| Overall design |
We cultured glioblastoma cell U251 in normal culture or methionine deprivation culture for 48h, then extracted total RNA and detected it using Arraystar Human LncRNA microarray.
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| Contributor(s) |
Li J, Liu R, Hu H, Huang Y, Shi Y, Li H, Chen H, Cai M, Wang N, Yan T, Wang K, Liu H |
| Citation(s) |
39572759 |
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| Submission date |
Nov 18, 2024 |
| Last update date |
Nov 22, 2024 |
| Contact name |
Hong Hu |
| E-mail(s) |
[email protected]
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| Phone |
15685782660
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| Organization name |
Harbin medical university
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| Street address |
youzheng street
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| City |
Harbin |
| State/province |
黑龙江 |
| ZIP/Postal code |
150001 |
| Country |
China |
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| Platforms (1) |
| GPL26963 |
Agilent-085982 Arraystar human lncRNA V5 microarray |
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| Samples (6)
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| GSM8637854 |
Glioblastoma U251 methionine normal culture, biological rep1 |
| GSM8637855 |
Glioblastoma U251 methionine normal culture, biological rep2 |
| GSM8637856 |
Glioblastoma U251 methionine normal culture, biological rep3 |
| GSM8637857 |
Glioblastoma U251 methionine deprived culture 48h, biological rep1 |
| GSM8637858 |
Glioblastoma U251 methionine deprived culture 48h, biological rep2 |
| GSM8637859 |
Glioblastoma U251 methionine deprived culture 48h, biological rep3 |
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| Relations |
| BioProject |
PRJNA1187564 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE282194_RAW.tar |
18.3 Mb |
(http)(custom) |
TAR (of TXT) |
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