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| Status |
Public on Nov 25, 2024 |
| Title |
Effect of deletion of UTX expression in NY8.3 progenitor CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Type 1 diabetes (T1D) is a chronic autoimmune disease resulting from the destruction of insulin-producing beta cells. This persistence is due to the continual replenishment of short-live effector CD8+ T cells (autoimmune mediators, Tmed) in the pancreatic lymph nodes (pLNs) by a long-lived reservoir of stem-like memory CD8+ T cells (autoimmune progenitors, Tprog). We are investigating an epigenetic regulator UTX playing the role in Tprog to Tmed conversion. The NOD mice with T cell-specific UTX deficiency (UTXTCD mice) were protected from T1D. In addition, the deletion of UTX in CD8+ T cells resulted in the accumulation of Tprog and loss of Tmed populations in pLNs. The function of UTX is to demethylate the histone mark, H3K27 tri-methylation, leading to the open chromatin accessibility for gene expression. ATAC-seq and RNA-seq of antigen specific Tprog cells revealed a role of UTX in closing chromatin at progenitor gene loci while enforcing accessibility at effector gene loci. Taken together, these findings point to the potential of targeting UTX-mediated conversion of Tprog into Tmed for interrupting the T1D autoimmune response.
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| Overall design |
To investigate the gene expression regulated by UTX, we sorted the Ly108+CXCR6- CD8+ T cells harvested from pancreatic lymph node of NOD.NY8.3 (NY8.3) mice and T cell specific deletion UTX deficient NY8.3 mice (UTXTCD.NY8.3). We then perform gene expression profiling using RNA-seq. There are 3 samples for each group (NY8.3 and UTXTCD.NY8.3)
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| Contributor(s) |
Chen H, Zhang S, Kohn L, Wang H, Sailer D, McCarthy E, Seyedsadr M, Zhou Z, Yin X, Wilkinson N, Lechner M, Hugo W, Su M |
| Citation missing |
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| Submission date |
Nov 18, 2024 |
| Last update date |
Nov 25, 2024 |
| Contact name |
Ho Chung Chen |
| E-mail(s) |
[email protected]
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| Phone |
9088127556
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| Organization name |
University of California, Los Angeles
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| Department |
MIMG
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| Lab |
Maureen Su
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| Street address |
609 Charles E Young Dr E
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90024 |
| Country |
USA |
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| Platforms (1) |
| GPL34290 |
Illumina NovaSeq X Plus (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1187616 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE282227_normalized_counts.txt.gz |
980.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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