|
| Status |
Public on Nov 25, 2024 |
| Title |
UTX_CD8+ Progenitor_1 |
| Sample type |
SRA |
| |
|
| Source name |
pancreatic lymph node
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreatic lymph node
cell type: CD8+ T cells
genotype: UTX_knockout
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated using Quick-RNA MicroPrep (Zymo)
RNA libraries for RNA-seq were prepared using KAPA mRNA HyperPrep Kit following manufacturer's protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq X Plus |
| |
|
| Description |
UTX1
|
| Data processing |
sequence reads were passed the QC test and were trimmed for adaptor sequence/low quality seuqence.
Trimmed sequence reads were mapped to mm10 using HISAT2 (2.2.1) on Galaxy to generate the bam file.
raw count table was generated by using featureCounts.
Raw count table was analzyed by Deseq2 to generate the normalized count table.
Assembly: mm10
Supplementary files format and content: tab-delimited text file includes normalized count with p-value and fold change for each sample
|
| |
|
| Submission date |
Nov 18, 2024 |
| Last update date |
Nov 25, 2024 |
| Contact name |
Ho Chung Chen |
| E-mail(s) |
[email protected]
|
| Phone |
9088127556
|
| Organization name |
University of California, Los Angeles
|
| Department |
MIMG
|
| Lab |
Maureen Su
|
| Street address |
609 Charles E Young Dr E
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90024 |
| Country |
USA |
| |
|
| Platform ID |
GPL34290 |
| Series (1) |
| GSE282227 |
Effect of deletion of UTX expression in NY8.3 progenitor CD8+ T cells |
|
| Relations |
| BioSample |
SAMN44800733 |
| SRA |
SRX26758907 |
