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| Status |
Public on Aug 20, 2011 |
| Title |
Gene expression patterns in response to GATA2 (WT and mutants) in HL-60 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
GATA2 mutants were discovered in families predisposed to MDS/AML and in sporadic cases of CML-blast crisis. Promyelocytic HL-60 cells were transduced with lentiviral vectors that express GATA2 WT or T354M, 355delT or L359V mutants upon addition of 4-hydroxy tamoxifen (4HT). Microarrays were performed to identify GATA2 WT signatures and differences caused by these mutations.
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| Overall design |
HL-60 cells transduced with GATA2 (WT, T354M, 355delT and L359V) lentiviruses were treated with 4HT for 24 hours and RNA isolated and gene expression measured on Affymetrix microarrays.
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| Contributor(s) |
Hahn CN, Kok CH, Chong C |
| Citation(s) |
21892162 |
| |
| Submission date |
May 13, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Chung H Kok |
| E-mail(s) |
[email protected]
|
| Organization name |
South Australia Health and Medical Research Institute
|
| Street address |
North Terrace
|
| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA139931 |
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