|
| Status |
Public on Aug 20, 2011 |
| Title |
HL-60-T354M_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HL-60 cells constitutively expressing GAL4-ER-VP16 tripartitie fusion protein and induced expression of GATA2 T354M
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HL-60
cell type: Promyelocytic Leukaemia
genotype/variation: constitutively expressing GAL4-ER-VP16 tripartitie fusion protein and induced expression of GATA2 T354M
|
| Treatment protocol |
24 hours prior to 4HT addition, the cells were changed into fresh RPMI medium containing 5% FBS with 10 µg/ml gentamicin and without hygromycin and puromycin. HL-60 cell lines were then treated with 100nM 4HT for 24 hours and, after this time, cells were harvested and RNA purified.
|
| Growth protocol |
HL-60 cell lines were grown in RPMI containing 10%FBS (Sigma-Aldrich), 500 µg/ml hygromycin, 3 µg/ml puromycin, 10 µg/ml gentamicin at density of 1-5x106/ml. 24 hours prior to 4HT addition, the cells were changed into fresh RPMI medium containing 5% FBS with 10 µg/ml gentamicin and without hygromycin and puromycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the RNeasy protocol (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Total RNA was converted to biotinylated sense DNA as per the Affymetrix Genechip Whole Transcript (WT) Sense Target Labeling Assay Manual.
|
| |
|
| Hybridization protocol |
Genechips were hybridised overnight at 45 degrees C for 17 hours in an Affymetrix Hybridisation oven.
|
| Scan protocol |
Genechips were scanned using the Affymetrix GSC3000 scanner.
|
| Data processing |
Raw CEL files were normalised with RMA method using aroma.affymetrix and the batch effect was removed using ComBat analysis.
|
| |
|
| Submission date |
May 13, 2011 |
| Last update date |
Aug 20, 2011 |
| Contact name |
Chung H Kok |
| E-mail(s) |
[email protected]
|
| Organization name |
South Australia Health and Medical Research Institute
|
| Street address |
North Terrace
|
| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29276 |
Gene expression patterns in response to GATA2 (WT and mutants) in HL-60 cells |
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