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| Status |
Public on May 01, 2012 |
| Title |
Differential regulation of Twist1-responsive genes in 4T1 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Twist1 variants including wildtype Twist1, a non-phosphorylatable mutant Twist1/S42A and a phospho-mimicking mutant Twist1/S42D were expressed in 4T1 cells in which the endogenous Twist1 was depleted.
We wanted to use microarray analysis to evaluate those genes that are differentially regulated by Twist1 variants.
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| Overall design |
All cell lines were grown to 70% confluency and total RNA was isolated with Qiagen kit. Each sample was prepared as triplicates. Parental 4T1 cells were used as control.
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| Contributor(s) |
Xue G, Restuccia DF |
| Citation(s) |
22585995 |
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| Submission date |
Jun 06, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Gongda Xue |
| E-mail(s) |
[email protected]
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| Organization name |
Friedrich Miescher Institute for Biomedical Research
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| Street address |
Maulbeerstrasse 66
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| City |
Basel |
| ZIP/Postal code |
CH-4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (15)
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| GSM737563 |
4T1_Tw1KD cells, rep1 |
| GSM737564 |
4T1_Tw1KD cells, rep2 |
| GSM737565 |
4T1_Tw1KD cells, rep3 |
| GSM737566 |
4T1_Tw1KD/Twist1, rep1 |
| GSM737567 |
4T1_Tw1KD/Twist1, rep2 |
| GSM737568 |
4T1_Tw1KD/Twist1, rep3 |
| GSM737569 |
4T1_Tw1KD/Twist1/S42A, rep1 |
| GSM737570 |
4T1_Tw1KD/Twist1/S42A, rep2 |
| GSM737571 |
4T1_Tw1KD/Twist1/S42A, rep3 |
| GSM737572 |
4T1_Tw1KD/Twist1/S42D, rep1 |
| GSM737573 |
4T1_Tw1KD/Twist1/S42D, rep2 |
| GSM737574 |
4T1_Tw1KD/Twist1/S42D, rep3 |
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| Relations |
| BioProject |
PRJNA140965 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29754_RAW.tar |
64.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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