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| Status |
Public on Dec 31, 2011 |
| Title |
Expression data from rice after brown planthopper attack |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of this study was to analyze potential brown planthopper (BPH) resistant genes in Rathu Heenati (RHT) by Affymetrix whole rice genome array,BPH susceptible and resistant rice varieties of TN1（Taichung Native 1）as control. All the resistant related genes derived from RHT will be analyzed according to the SSR markers interval flanked on the chromosome 3, 4, 6 and 10. It will be benefit to the gene clone and marker assistant breeding for Bph3 gene in the near future.
We used microarrays to detail the global differential gene expression before and after brown planthopper attack in two different varieties, and identified distinct classes of high enriched genes induced by BPH or constituent in RHT
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| Overall design |
The 2nd to 3rd instar nymphs of BPH were transferred to tillering stage seedings (10 BPH nymphs per plant) in a box covered with nylon-mesh. Stems of the rice plant infected by BPH were collected at 0h (T0), 8h (T8), 24h (T24) after BPH attack, total RNA were extracted for the microarray hybirdlization.
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| Contributor(s) |
Miao X, Wang Y |
| Citation(s) |
22083132 |
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| Submission date |
Jun 14, 2011 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Yubing Wang |
| E-mail(s) |
[email protected]
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| Phone |
86-24-54924217
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| Organization name |
Institute of Plant Physiology and Ecology
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| Street address |
300 fenglin road
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| City |
shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (18)
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| GSM741675 |
RHT stem at T8, biological rep1 |
| GSM741676 |
RHT stem at T8, biological rep2 |
| GSM741677 |
RHT stem at T8, biological rep3 |
| GSM741678 |
RHT stem at T24, biological rep1 |
| GSM741679 |
RHT stem at T24, biological rep2 |
| GSM741680 |
RHT stem at T24, biological rep3 |
| GSM741681 |
TN1 stem at T0, biological rep1 |
| GSM741682 |
TN1 stem at T0, biological rep2 |
| GSM741683 |
TN1 stem at T0, biological rep3 |
| GSM741684 |
TN1 stem at T8, biological rep1 |
| GSM741685 |
TN1 stem at T8, biological rep2 |
| GSM741686 |
TN1 stem at T8, biological rep3 |
| GSM741687 |
TN1 stem at T24, biological rep1 |
| GSM741688 |
TN1 stem at T24, biological rep2 |
| GSM741689 |
TN1 stem at T24, biological rep3 |
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| Relations |
| BioProject |
PRJNA140845 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29967_RAW.tar |
77.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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