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| Status |
Public on Sep 01, 2011 |
| Title |
Expression data from Tnrc6a (GW182) mutant yolk sac |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
GW182 (Tnrc6a) is a key component of RISC (miRNA-Induced Silencing Complex) that plays a critical role in miRNA-mediated gene silencing. Here, we show that GW182 is expressed in the yolk sac endoderm, and that gene-trap disruption of GW182 leads to growth arrest of yolk sac endoderm, impaired hematopoiesis and embryonic lethality.
To investigate roles of GW182 in the yolk sac endoderm, we assessed changes in mRNA expression in the yolk sac of E9.5 GW182gt/gt embryos using microarrays (Affymetrix).
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| Overall design |
Yolk sac of wild type littermates and GW182gt/gt embryos at E9.5 was collected for total RNA isolation using Trizol (Invitrogen). RNAs were purified according to the manufacturer’s protocol before subjected to Mouse Gene 1.0 ST Whole Genome Array (Affymetrix) for mRNA expression profiling. Experiments were performed in triplicate. Differentially expressed mRNAs were identified using a two-sample t-test (P<0.05 considered significant).
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| Contributor(s) |
Lu J, Jiang Z |
| Citation(s) |
22187428, 37127654 |
| |
| Submission date |
Jun 27, 2011 |
| Last update date |
Aug 02, 2023 |
| Contact name |
jining lu |
| E-mail(s) |
[email protected]
|
| Phone |
617-638-6180
|
| Fax |
617-536-8093
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| Organization name |
Boston University
|
| Street address |
715 Albany street R304
|
| City |
BoSTON |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM748906 |
Tnrc6a(GW182) mutant mouse E9.5 yolk sac rep1 |
| GSM748907 |
Tnrc6a(GW182) mutant mouse E9.5 yolk sac rep2 |
| GSM748908 |
Tnrc6a(GW182) mutant mouse E9.5 yolk sac rep3 |
|
| Relations |
| BioProject |
PRJNA143773 |
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