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| Status |
Public on Jul 23, 2011 |
| Title |
H4IIE liver cell line ChIP-Chip with Egr1 |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Egr1 regulates genes involved in cholesterol biosynthesis in liver. ChIP-chip assays were performed on H4IIE liver cells that were either treated or untreated with insulin for one hour.
This experiment includes a custom ChIP-chip design incorporating many genes that are dynamically regulated by insulin signaling. The Egr1 antibody used was from Santa Cruz sc-189.
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| |
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| Overall design |
Egr1 ChIP samples from untreated and 1h insulin treated cells were hybridized.
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| Contributor(s) |
Svaren J, Gokey N |
| Citation(s) |
21712389 |
| |
| Submission date |
Jul 20, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
John Svaren |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Wisconsin
|
| Department |
Comparative Biosciences
|
| Lab |
Waisman Center
|
| Street address |
1500 Highland Ave.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL10812 |
UW_NimbleGen_Rat_321K_Array design 081105 |
| GPL13957 |
UW Nimblegen Rattus norvegicus custom array design 4845 |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA144193 |
