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| Status |
Public on Apr 09, 2012 |
| Title |
The Histone Methyltransferase Wbp7 Controls Macrophage Function through GPI Glycolipid Anchor Synthesis. [Expression Profile] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Histone methyltransferases catalyze site-specific deposition of methyl groups, enabling recruitment of transcriptional regulators. In mammals, trimethylation of lysine 4 in histone H3, a modification localized at the transcription start sites of active genes, is catalyzed by six enzymes (SET1a and SET1b, MLL1–MLL4) whose specific functions are largely unknown. By using a genomic approach, we found that in macrophages, MLL4 (also known as Wbp7) was required for the expression of Pigp, an essential component of the GPI-GlcNAc transferase, the enzyme catalyzing the first step of glycosylphosphatidylinositol (GPI) anchor synthesis. Impaired Pigp expression in Wbp7-/- macrophages abolished GPI anchor-dependent loading of proteins on the cell membrane. Consistently, loss of GPI-anchored CD14, the coreceptor for lipopolysaccharide (LPS)
and other bacterial molecules, markedly attenuated LPS-triggered intracellular signals and gene expression changes. These data link a histone-modifying enzyme to a biosynthetic pathway and indicate
a specialized biological role for Wbp7 in macrophage function and antimicrobial response.
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| Overall design |
Gene expression profiles for bone marrow-derived macrophages (BMDM) from either Wbp7+/- (HET) or Wbp7-/- (KO). Cells were left untreated or challenged with lipopolysaccharide (LPS) for 2hrs or 4hrs. Each genotype-treatment combination were performed in triplicate.
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| Contributor(s) |
Austenaa L, Barozzi I, Chronowska A, Termanini A, Ostuni R, Prosperini E, Stewart F, Testa G, Natoli G |
| Citation(s) |
22483804 |
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| Submission date |
Jul 27, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Iros Barozzi |
| E-mail(s) |
[email protected]
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| Organization name |
Medical University Vienna
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| Street address |
Borschkegasse 8a
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| GSM767719 |
Wbp7+/-, untreated, replicate 2 |
| GSM767720 |
Wbp7+/-, LPS 2hrs, replicate 2 |
| GSM767721 |
Wbp7+/-, LPS 4hrs, replicate 2 |
| GSM767722 |
Wbp7+/-, untreated, replicate 3 |
| GSM767723 |
Wbp7+/-, LPS 2hrs, replicate 3 |
| GSM767724 |
Wbp7+/-, LPS 4hrs, replicate 3 |
| GSM767725 |
Wbp7-/-, untreated, replicate 1 |
| GSM767726 |
Wbp7-/-, LPS 2hrs, replicate 1 |
| GSM767727 |
Wbp7-/-, LPS 4hrs, replicate 1 |
| GSM767728 |
Wbp7-/-, untreated, replicate 2 |
| GSM767729 |
Wbp7-/-, LPS 2hrs, replicate 2 |
| GSM767730 |
Wbp7-/-, LPS 4hrs, replicate 2 |
| GSM767731 |
Wbp7-/-, untreated, replicate 3 |
| GSM767732 |
Wbp7-/-, LPS 2hrs, replicate 3 |
| GSM767733 |
Wbp7-/-, LPS 4hrs, replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE30973 |
The Histone Methyltransferase Wbp7 Controls Macrophage Function through GPI Glycolipid Anchor Synthesis |
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| Relations |
| BioProject |
PRJNA154633 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30971_RAW.tar |
81.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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