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| Status |
Public on Sep 16, 2011 |
| Title |
Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In response to microenvironmental signals macrophages undergo different activation, indicated as classic/M1 and alternative/M2 polarization. C-Myc transcription factor could be an essential player in M2 polarization.
Functional relevance of c-Myc in M2 macrophage biology is investigated by evaluating the effect of 100-58F4, on the transcriptional profile induced on human macrophages by IL-4.
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| Overall design |
Human monocytes were obtained from normal donor buffy coats by two-step gradient centrifugation using Ficoll (Biochrom) and Percoll (Amersham). Non-adherent cells were discarded, and the purified monocytes were incubated for 7 days in RPMI 1640 (Biochom) supplemented with 10% FCS (HyClone) and 100 ng/mL M-CSF to obtain resting macrophages. Macrophage polarization was obtained by removing the culture medium and culturing cells in RPMI 1640 supplemented with 10% FCS and 100 ng/mL LPS plus 20 ng/mL IFN-gamma (M1 polarization) or 20 ng/mL IL-4 (M2 polarization) for 24 h. When needed, chemical inhibitors were added with IL-4.
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| Contributor(s) |
Pello OM |
| Citation(s) |
22067385 |
| |
| Submission date |
Sep 15, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Massimo Locati |
| E-mail(s) |
[email protected]
|
| Phone |
+39 02 8224 5116
|
| Fax |
02-82245101
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| URL |
http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
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| Organization name |
Istituto Clinico Humanitas
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| Department |
Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
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| Lab |
Laboratory of Leukocyte Biology
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| Street address |
Via Manzoni, 113
|
| City |
Rozzano |
| State/province |
MILAN |
| ZIP/Postal code |
20089 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (9)
|
| GSM797441 |
Resting fully differentiated macrophages (7 days of culture), rep 1 |
| GSM797442 |
Alternative activated macrophages (M2) rep 1 |
| GSM797443 |
Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 1 |
| GSM797444 |
Resting fully differentiated macrophages (7 days of culture), rep 2 |
| GSM797445 |
Alternative activated macrophages (M2) rep 2 |
| GSM797446 |
Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 2 |
| GSM797447 |
Resting fully differentiated macrophages (7 days of culture), rep 3 |
| GSM797448 |
Alternative activated macrophages (M2) rep 3 |
| GSM797449 |
Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 3 |
|
| Relations |
| BioProject |
PRJNA147641 |
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