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Series GSE33756 Query DataSets for GSE33756
Status Public on Nov 17, 2011
Title tRNAs marked with CCACCA are targeted for degradation
Organism Saccharomyces cerevisiae
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The CCA-adding enzyme adds CCA to the 3' ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3' ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. Here, we report deep sequencing analysis of the 3' ends of tRNA-Ser-CGA and tRNA-Ser-UGA from S. cerevisiae strains and show that hypomodified mature tRNAs are subjected to CCACCA (or poly(A) addition) as part of a rapid tRNA decay pathway in vivo. We conjecture that CCACCA addtion is a universal mechanism for controlling tRNA levels and preventing errors in translation.
 
Overall design 121 samples analyzed in total, representing time courses of 10 different yeast strains; Biological replicates for each time point are included
 
Contributor(s) Wilusz JE, Whipple JM, Phizicky EM, Sharp PA
Citation(s) 22076379
Submission date Nov 16, 2011
Last update date May 15, 2019
Contact name Jeremy E Wilusz
E-mail(s) [email protected]
Phone 617-253-6457
Fax 617-253-3867
Organization name Massachusetts Institute of Technology
Department Koch Institute for Integrative Cancer Research
Street address 77 Massachusetts Ave, Bldg 76-461
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (1)
GPL13821 Illumina HiSeq 2000 (Saccharomyces cerevisiae)
Samples (121)
GSM834995 WT(JMW007) 0 hr Replicate 1
GSM834996 WT(JMW007) 0 hr Replicate 2
GSM834997 WT(JMW007) 0 hr Replicate 3
Relations
SRA SRP009362
BioProject PRJNA148403

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Supplementary file Size Download File type/resource
GSE33756_RAW.tar 4.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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