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| Status |
Public on Jan 01, 2013 |
| Title |
Expression data from mesodermal Tbx1-knockout otic vesicles and periotic mesenchyme at E11.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The T-box transcription factor Tbx1 is expressed in the otic vesicle and surrounding periotic mesenchyme during inner ear development. Mesenchymal Tbx1 is essential for inner ear development, with conditional mutants displaying defects in both auditory and vestibular systems. We have previously identified reduced expression of retinoic acid metabolic genes in the periotic mesenchyme of mesoderm conditional Tbx1 mutants, using the T-Cre mouse line, implicating retinoic acid in mesenchymal-epithelial signaling downstream of Tbx1 in the periotic mesenchyme.
In order to identify downstream effectors of mesenchymal-epithelial signaling downstream of mesenchymal Tbx1, we have utilized a gene profiling approach comparing embryonic day 11.5 otic vesicle and surrounding periotic mesenchyme from T-Cre-mediated conditional Tbx1 mutants (Mest-KO) and conditional heterozygous control litter mates (control). This data was used in conjunction with OV-only data (GSE34064) to identify expression changes within the periotic mesenchyme.
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| Overall design |
E11.5 T-Cre-mediated conditional Tbx1 mutants (Mest-KO) and control (T-Cre conditional Tbx1 heterozygotes) embryos were microdissected to isolate the otic vesicle and surrounding periotic mesenchyme. Left and right ears from 3 embryos of the same genotype were pooled for each chip, and 3 biological replicates of each chip were performed.
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| Contributor(s) |
Monks DC, Braunstein EM, Morrow BE |
| Citation missing |
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| |
| Submission date |
Nov 30, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Tingwei Guo |
| E-mail(s) |
[email protected]
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| Phone |
(718) 678-1122
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| Organization name |
Albert Einstein College of Medicine
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| Department |
Genetics
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| Lab |
Morrow Lab
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| Street address |
1301 Morris Park Avenue, Room 408
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| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM841210 |
T-Cre conditional Tbx1 knockout otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 1 |
| GSM841211 |
T-Cre conditional Tbx1 knockout otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 2 |
| GSM841212 |
T-Cre conditional Tbx1 knockout otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 3 |
| GSM841213 |
T-Cre conditional Tbx1 heterozygous control otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 1 |
| GSM841214 |
T-Cre conditional Tbx1 heterozygous control otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 2 |
| GSM841215 |
T-Cre conditional Tbx1 heterozygous control otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE34066 |
Expression data from mesodermal Tbx1-knockout ears, otic vesicles, and periotic mesenchyme |
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| Relations |
| BioProject |
PRJNA156513 |
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