|
| Status |
Public on Jan 01, 2013 |
| Title |
T-Cre conditional Tbx1 knockout otic vesicle and periotic mesenchyme tissue at E11.5, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
E11.5 otic vesicle and periotic mesenchyme, T-Cre conditional Tbx1 knockout
|
| Organism |
Mus musculus |
| Characteristics |
age: E11.5
genotype: T-Cre conditional Tbx1 knockout
tissue: microdissected otic vesicle and periotic mesenchyme
|
| Growth protocol |
Timed matings were dissected at embryonic day 11.5, and periotic tissue was isolated, including otic vesicle and perotic mesenchyme. RNA was isolated from the tissue immediately following dissection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Micro kit according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to Affymetrix protocols from 300ng of total RNA.
|
| |
|
| Hybridization protocol |
10ug of cRNA was hybridized for 16hrs at 45C on Affymetrix Mouse 430_2 arrays.
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip 7G Scanner.
|
| Description |
MestKO1_OVPOM
3 pooled embryos.
Gene expression data from E11.5 otic vesicles and periotic mesenchyme at the stage of dorsoventral elongation.
|
| Data processing |
Data was analyzed using Affymetrix Expression Console software version 1.1 using Affymetrix default protocols.
|
| |
|
| Submission date |
Nov 30, 2011 |
| Last update date |
Jan 01, 2013 |
| Contact name |
Tingwei Guo |
| E-mail(s) |
[email protected]
|
| Phone |
(718) 678-1122
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Genetics
|
| Lab |
Morrow Lab
|
| Street address |
1301 Morris Park Avenue, Room 408
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE34065 |
Expression data from mesodermal Tbx1-knockout otic vesicles and periotic mesenchyme at E11.5 |
| GSE34066 |
Expression data from mesodermal Tbx1-knockout ears, otic vesicles, and periotic mesenchyme |
|
