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| Status |
Public on Oct 28, 2005 |
| Title |
In situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used to assess the accuracy and precision of the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection.
Keywords: dye swap design,quality control testing design
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| Overall design |
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used to assess the accuracy and precision of the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection.
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| Contributor(s) |
Carter MG, Hamatani T, Sharov AA, Carmack CE, Qian Y, Aiba K, Ko NT, Dudekula DB, Brzoska PM, Hwang SS, Ko MS |
| Citation(s) |
12727912 |
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| Submission date |
Oct 26, 2005 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Minoru S.H. Ko |
| E-mail(s) |
[email protected]
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| Phone |
410-558-8359
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| Organization name |
NIH
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| Department |
National Institute on Aging
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| Lab |
Lab of Genetics
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| Street address |
251 Bayview Blvd, Suite 100, 10C
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL870 |
NIA Mouse 22K Microarray v1.0 (Development 60-mer Oligo) |
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| Samples (6)
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| GSM80039 |
E12.5 Embryo3, 3 pooled(16011321000018) |
| GSM80040 |
E12.5 Placenta3, 3 pooled(16011321000019) |
| GSM80043 |
E12.5 Embryo1, 5 pooled(16011321000040) |
| GSM80044 |
E12.5 Placenta1, 5 pooled(16011321000041) |
| GSM80184 |
E12.5 Embryo2, 5 pooled(16011321000044) |
| GSM80185 |
E12.5 Placenta2, 5 pooled(16011321000045) |
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| Relations |
| BioProject |
PRJNA93563 |
| Supplementary data files not provided |
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