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| Status |
Public on Jul 01, 2012 |
| Title |
Genome-wide analysis of gene expression in WT and Erk2 deficient CD4 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Effective immune responses depend upon appropriate T cell differentiation in accord with the nature of an infectious agent, and the contingency of differentiation depends minimally on T cell antigen receptor, co-receptor, and cytokine signals. In this reverse genetic study we show that the Map Kinase, Erk2, is nonessential for T cell proliferation in the presence of optimum co-stimulation. Instead, it has opposite polar effects on T-bet and Gata3 expression and hence on Th1 and Th2 differentiation. Alternatively, in the presence of TGFbeta, the Erk pathway suppresses a large program of gene expression effectively limiting the differentiation of Foxp3+ T reg cells. In the latter case, the mechanisms involved include suppression of Gata3 and Foxp3, induction of Tbx21, phosphorylation of Smad2,3, and possibly suppression of Socs2, a positive inducer of Stat5 signaling. Consequently, loss of Erk2 severely impeded Th1 differentiation while enhancing the development of Foxp3+ induced T regulatory cells. Selected profiles of gene expression under multiple conditions of T cell activation illustrate the opposing consequences of Erk pathway signaling.
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| Overall design |
Analysis of the Th1 and iTreg differentiation regulated by Erk2.
Total RNA obtained from WT or Erk2 deficient CD4 T cells in different conditions.
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| Contributor(s) |
Hedrick SM, Chang CF |
| Citation(s) |
22675204 |
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| Submission date |
Apr 24, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Chiungfang Chang |
| E-mail(s) |
[email protected]
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| Phone |
858-534-6559
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| Fax |
858-534-0980
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| Organization name |
University of California, San Diego
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| Department |
Biological Science
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| Lab |
NSB 5116
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92122 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA161521 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE37554_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE37554_non-normalized_data.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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