|
| Status |
Public on Jul 01, 2012 |
| Title |
5388876092_F |
| Sample type |
RNA |
| |
|
| Source name |
Erk2 deficient 3hr iTreg
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
cell type: CD4 T cells
genotype/variation: Erk2 KO
culture conditions: iTreg
time: 3hr
|
| Growth protocol |
CD4 T cells were cultured under TH0-, Th1-, or iTreg-inducing conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
KO 3hr iTreg
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 24, 2012 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Chiungfang Chang |
| E-mail(s) |
[email protected]
|
| Phone |
858-534-6559
|
| Fax |
858-534-0980
|
| Organization name |
University of California, San Diego
|
| Department |
Biological Science
|
| Lab |
NSB 5116
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92122 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE37554 |
Genome-wide analysis of gene expression in WT and Erk2 deficient CD4 T cells |
|
