|
| Status |
Public on Aug 07, 2012 |
| Title |
Genome-wide analysis of macrophage gene expression upon Bcl3 knockdown |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
By binding to specific DNA elements, known collectively as “κB sites”, contained within the promoters/enhancers of target genes, NF-κB regulates gene expression. We found that the identity of the central base pair (bp) of κB sites profoundly impacts the transcriptional activity of NF-κB dimers. RelA dimers prefer an A/T bp at this position for optimum transcriptional activation (A/T-centric) and discriminate against G/C-centric κB sites. The p52 homodimer, in contrast, activates transcription from G/C-centric κB sites in complex with Bcl3 but represses transcription from the A/T-centric sites. The p52:Bcl3 complex binds to these two classes of κB sites in distinct modes permitting recruitment of coactivator, corepressor, or both coactivator and corepressor complexes in promoters containing G/C, A/T or both G/C and A/T-centric sites. Therefore, through sensing of bp differences within κB sites, NF-κB dimers modulate biological programs by activating, repressing and altering expression of effector genes.
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| Overall design |
Total RNA extracted from bone marrow derived macrophages (BMDMs) with Bcl3 siRNA knockdown or mouse scramble siRNA knockdown were subjected to LPS stimulation.
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| |
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| Contributor(s) |
Wang VY, Ghosh G, Hoffmann A |
| Citation(s) |
23063365 |
| |
| Submission date |
Aug 07, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Vivien Ya-Fan Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSD
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Gourisankar Ghosh Lab
|
| Street address |
9500 Gilman Drive, NSB, 3325A
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA172063 |
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