|
| Status |
Public on Aug 07, 2012 |
| Title |
BMDM siCTL 0hr LPS #2 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM siCTL 0hr LPS (no stimulation)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 5-7 weeks
tissue: bone marrow
cell type: bone marrow derived macrophage (BMDM)
transfected with: scramble siRNA (control)
|
| Treatment protocol |
BMDMs were transfected with siRNA and then stimulated with LPS
|
| Growth protocol |
BMDMs were cultured in RPMI 1640 medium supplemented with 25% FBS and 25% L cell-conditioned medium as a source of M-CSF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted QIAGEN RNeasy kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Strepavidin Cy-3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 2
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with Illumina Inc. BeadStudio (v.2010.3.0.30128).
|
| |
|
| Submission date |
Aug 07, 2012 |
| Last update date |
Aug 07, 2012 |
| Contact name |
Vivien Ya-Fan Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSD
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Gourisankar Ghosh Lab
|
| Street address |
9500 Gilman Drive, NSB, 3325A
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE39922 |
Genome-wide analysis of macrophage gene expression upon Bcl3 knockdown |
|
