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| Status |
Public on Aug 31, 2012 |
| Title |
Identification of target genes of the DELLA protein RGL2 during the repression of seed germination |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
To shed light on the genetic events downstream of DELLA proteins, we have employed a microarray expression profiling of Arabidopsis thaliana seeds to identify target genes of the DELLA protein RGL2. Seeds of the germinating ga1-3 rga-t2 rgl2-1 and the non-germinating ga1-3 rga-t2 mutant were stratified, and RNA was extracted after five days. Transcript profiles of the non-germinating seeds closely resemble profiles of dormant seeds, and several transcription factors involved in light- and phytohormone-regulated signalling pathways appear to be up-regulated, suggesting that RGL2 controls various physiological aspects to inhibit seed germination.
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| Overall design |
Gene expression five days after stratification was measured in ga1-3 rga-t2 seeds, using seeds of the ga1-3 rga-t2 rgl2-1 mutant as reference. Two biological replicates were performed for each sample.
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| Contributor(s) |
Stamm P, Ravindran P, Mohanty B, Tan EL, Yu H, Kumar PP |
| Citation(s) |
23035751 |
| |
| Submission date |
Aug 30, 2012 |
| Last update date |
Apr 22, 2015 |
| Contact name |
Petra Stamm |
| E-mail(s) |
[email protected]
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| Organization name |
National Univerisity of Singapore
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| Department |
Department of Biological Sciences
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| Lab |
Plant Morphogenesis Lab
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| Street address |
Science Drive 4
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| City |
Singapore |
| ZIP/Postal code |
117543 |
| Country |
Singapore |
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| Platforms (1) |
| GPL12621 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA174131 |
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