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| Status |
Public on Feb 26, 2013 |
| Title |
Expression data from wild-type and microRNA-155 (miR-155) deficient CD8 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNA-155 (miR-155) is upregulated in primary effector CD8 T cells but is expressed at low amounts in naïve cells. Anti-viral CD8 T cell responses and viral clearance were impaired in miR-155 deficient (bic-/-) mice, and this defect was intrinsic to CD8 T cells, as adoptively transferred bic-/- CD8 T cells generated greatly reduced primary and memory responses during infection. To understand the mechanism by which miR-155 regulates CD8 T cell activation, we analyzed the gene expression profiles of naive and in vitro activated wild-type and bic-/- CD8 T cells.
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| Overall design |
CD8 T cells were purified from uninfected C57BL/6 mice and stimulated in vitro with plate-bound anti-CD3 and anti-CD28 antibodies for 48 h or left unstimulated. RNA from these CD8 T cells was processed, amplified, labeled, and hybridized to Affymetrix GeneChip MoGene 1.0 st microarrays.
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| Contributor(s) |
Wherry EJ, Stelekati E, Gracias DT, Doering TA, Katsikis PD |
| Citation(s) |
23603793 |
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| Submission date |
Feb 25, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
E John Wherry |
| Organization name |
University of Pennsylvania
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| Street address |
421 Curie Blvd.
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| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA190692 |
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