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| Status |
Public on Oct 09, 2014 |
| Title |
Beta-tubulin and Actin Filament-Associating Small GTPase GIMAP4 is Required for IFN-Ɣ Secretion During Early Human CD4+ T Cell Activation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the dataset was to study on a genome-wide level the effect of GTPase of the human immune associated protein 4 (GIMAP4) knockdown on the gene expression of resting T cells and immediately after T cell activation and Th1(Act+IL12) polarizing conditions of human cord blood-derived CD4+ T cells.
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| Overall design |
Total RNA from GIMAP4 siRNA-treated cord blood CD4+ T cells 48 hours after siRNA treatment, before TCR activation and corresponding cells cultured for 2h in TCR activating (antiCD3+antiCD28) plus IL-12 conditions was compared to total RNA from nonspecific control siRNA-treated cells. Samples from 3 biological replicates were analysed.
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| Contributor(s) |
Heinonen M, Kanduri K, Lähdesmäki H, Lahesmaa R, Henttinen T |
| Citation(s) |
25287446 |
| |
| Submission date |
Apr 03, 2013 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Kartiek Kanduri |
| Phone |
+358-2-333-8001
|
| Organization name |
Turku Centre for Biotechnology
|
| Lab |
Molecular Systems Immunology / Lahesmaa
|
| Street address |
Tykistokatu 6
|
| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (12)
|
| GSM1112854 |
CordBlood_CD4+_GIMAP4_siRNA_rep1 |
| GSM1112855 |
CordBlood_CD4+_GIMAP4_siRNA_rep2 |
| GSM1112856 |
CordBlood_CD4+_GIMAP4_siRNA_rep3 |
| GSM1112857 |
CordBlood_CD4+_control_siRNA_Act_2h_rep1 |
| GSM1112858 |
CordBlood_CD4+_control_siRNA_Act_2h_rep2 |
| GSM1112859 |
CordBlood_CD4+_control_siRNA_Act_2h_rep3 |
| GSM1112860 |
CordBlood_CD4+_GIMAP4_siRNA_Act+IL12_2h_rep1 |
| GSM1112861 |
CordBlood_CD4+_GIMAP4_siRNA_Act+IL12_2h_rep2 |
| GSM1112862 |
CordBlood_CD4+_GIMAP4_siRNA_Act+IL12_2h_rep3 |
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| Relations |
| BioProject |
PRJNA196149 |
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