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| Status |
Public on Apr 13, 2013 |
| Title |
Expression data from Drosophila S2* cells treated with the steroid hormone 20-hydroxy-ecdysone (20E) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Previously we and other teams have found that 20E modulates the induction and expression of antimicrobial peptides (AMPs) in immune-challenged Drosophila cell culture or whole animals.
To identify potential downstream targets of 20E involved in modulating the immune response we used Affymetrix gene chips (microarrays) to compare the transcriptomes of Drosophila S2* cells treated or not with 20E for 24 hours.
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| Overall design |
Affymetrix Drosophila 2.0 Chips were probed in triplicate with RNA isolated from untreated S2* cells or cells treated with 1 microMolar 20E (Sigma) for 24 hours. cDNA products were hybridized at the Brown University Genomics Core Facility to Affymetrix GeneChip Drosophila_2.0 Genome Arrays (3 replicate chips per treatment).
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| Contributor(s) |
Rus F, Flatt T, Aggarwal K, Tong M, Okuda K, Kleino A, Yates E, Tatar M, Silverman N |
| Citation(s) |
23652443 |
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| Submission date |
Apr 12, 2013 |
| Last update date |
May 04, 2018 |
| Contact name |
Thomas Flatt |
| E-mail(s) |
[email protected]
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| Organization name |
University of Lausanne
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| Department |
Department of Ecology and Evolution
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| Street address |
UNIL Sorge, Biophore
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA196913 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46020_RAW.tar |
14.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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