|
| Status |
Public on Apr 13, 2013 |
| Title |
S2* cells, with 20E, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
S2* cells_1 µM of 20E for 24 hrs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: S2* cells
treated with: 1 microMolar steroid hormone 20-hydroxy-ecdysone (20E) for 24hr
|
| Treatment protocol |
Cells were either treated with 1 microMolar 20-hydroxy-ecdysone (20E) (Sigma) for 24 hours or left untreated (control)
|
| Growth protocol |
Drosophila S2* cells were grown in Schneider's medium (Gibco) with 10% fetal bovine serum, 1% Gluta-MAX (Gibco), and 0.2% penicillin-streptomycin (Gibco) at 27C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
cDNA products were hybridized at the Brown University Genomics Core Facility to Affymetrix GeneChip Drosophila_2.0 Genome Arrays (3 replicate chips per treatment)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Scanner 3000 7G at the Brown University Genomics Core Facility
|
| Description |
B2_Drosophila_2.0
|
| Data processing |
Expression data were processed and analyzed with dCHIP software (http://www.hsph.harvard.edu/cli/complab/dchip/); raw data were normalized across chips by the invariant difference selection algorithm with gene expression given by the model-based expression index.
|
| |
|
| Submission date |
Apr 12, 2013 |
| Last update date |
Apr 13, 2013 |
| Contact name |
Thomas Flatt |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Lausanne
|
| Department |
Department of Ecology and Evolution
|
| Street address |
UNIL Sorge, Biophore
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE46020 |
Expression data from Drosophila S2* cells treated with the steroid hormone 20-hydroxy-ecdysone (20E) |
|
