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Series GSE4668 Query DataSets for GSE4668
Status Public on Apr 14, 2006
Title Expression data from hormone-starved MCF7/BUS cell culture
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To obtain comprehensive information on 17beta-estradiol (E2) sensitivity of genes that are inducible or suppressible by this hormone, we designed a method that determines ligand sensitivities of large numbers of genes using DNA microarray and a set of simple Perl computer scripts implementing the standard metric statistics, and employed it to characterize effects of low (0-100 pM) concentrations of E2 on the transcriptome profile of MCF7/BUS human breast cancer cells, whose E2 dose-dependent growth curve saturated with 100 pM E2. Evaluation of changes in mRNA expression for all genes covered by the DNA microarray indicated that, at a very low concentration (10 pM), E2 suppressed 3~5 times larger numbers of genes than it induced, whereas at higher concentrations (30-100 pM) it induced 1.5~2 times more genes than it suppressed. Using clearly defined statistical criteria, E2-inducible genes were categorized into several classes based on their E2 sensitivities. This approach of hormone sensitivity analysis revealed that expression of two previously reported E2-inducible autocrine growth factors, TGF-? and SDF-1, was not affected by 100 pM and lower concentrations of E2 but strongly enhanced by 10 nM E2, which was far higher than the concentration that saturated the E2 dose-dependent growth curve of MCF7/BUS cells. These observations suggested that biological actions of E2 are derived from expression of multiple genes whose E2 sensitivities differ significantly and, hence, dependent on the E2 concentration especially when it is lower than the saturating level, emphasizing the importance of characterizing the ligand dose-dependent aspects of E2 actions. (paper abstract)
Keywords: Dose Response, Time Course
 
Overall design MCF7/BUS cells were exposed to varying concentrations of 17beta-estradiol (E2) for 48 hours. Experiments were repeated five times independently.
In another experiment, MCF7/BUS cells were subjected to hormone starvation. At day 0, 1, and 2, RNA samples were isolated from cell culture. Experiments were repeated three times independently.
 
Citation(s) 14610279
Submission date Apr 13, 2006
Last update date Aug 10, 2018
Contact name Kathryn Coser
E-mail(s) [email protected]
Organization name Mass General Hospital Cancer Center
Department DNA Microarray Core
Lab Shioda
Street address Blg 149 13th St
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (34)
GSM105292 MCF7/BUS, 0 pM E2, 48 hours, biological replication 1
GSM105293 MCF7/BUS, 0 pM E2, 48 hours, biological replication 2
GSM105294 MCF7/BUS, 0 pM E2, 48 hours, biological replication 3
Relations
BioProject PRJNA96813

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