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| Status |
Public on Jun 01, 2013 |
| Title |
Expression data for resting and activated B and T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The IgH locus encodes for part of the antibody exposed by B cells and is important for the immune system. In B cells, one allele produces protein, the other must remain silenced. It was proposed that both alleles reside in different nuclear compartments and that this is important to maintain mono-allelic productivity. Here we challenge this concept. We provide detailed genome-wide contact maps, which show that IgH adopts different nuclear locations in immune versus other cells but also demonstrate that in B cells both alleles reside in the same environment. Nuclear positioning is therefore not important to maintain allelic exclusion.
We did a gene expression analysis on resting (Day 0) and in vitro activated (Day 4) B and T cells in triplicate
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| Overall design |
Splenic B cells and T cells were separately isolated from spleen, using magnetic activated cell sorting, and activated in vitro for 4 days
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| Contributor(s) |
Holwerda SB, van de Werken HG, de Almeida CR, Bergen IM, de Bruijn MW, Verstegen MJ, Simonis M, Splinter E, Wijchers PJ, Hendriks RW, de Laat W |
| Citation(s) |
23748562 |
| |
| Submission date |
May 13, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
sjoerd holwerda |
| E-mail(s) |
[email protected]
|
| Organization name |
Hubrecht Institute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE47129 |
Allele specific analysis of the immunoglobulin heavy chain locus |
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| Relations |
| BioProject |
PRJNA202870 |
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