 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 11, 2014 |
| Title |
Gene expression in whole lung and pulmonary macrophages reflects the dynamic pathology associated with airway surface dehydration [left lobe only] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Scnn1b-Tg mice overexpress the beta subunit of the epithelial sodium channel (Scnn1b) in airway Club cells. The general phenotype of these mice is described in three published manuscripts (Mall et al. 2004, Nature Medicine, 10(5):487-93; Mall et al. 2008, Am J Respir Crit Care Med. 177(7):730-42; Livraghi-Butrico et al. 2012, Physiol. Genomics 44(8):470-84; and Livraghi-Butrico et al. 2012, Mucosal Immunology 5(4):397-408). Briefly, overexpression of the Scnn1b transgene in airway Club cells leads to hyperabsorption of sodium from the airway surface liquid, which causes airway surface liquid and mucus dehydration, resulting in reduced mucus clearance and airway mucus obstruction. The data provided here represents mRNA expression data from disseccted whole lung from male WT and Scnn1b-transgenic littermates (C57Bl/6NTac background) at 4 time points [postnatal days (PND) 0, 3, 10, and 42]. Histologically, PND 0 lungs are normal, at PND 3 the intrapulmonary airways exhibit transient and spotty Club cell necrosis, and by PND 10 airway mucus obstruction is evident in the proximal portion of the intrapulmonary main stem bronchus. At PND 42, Scnn1b-Tg lungs are charactyerized by chronic low level inflammation, with activated macrophages, neutrophilia, eosinophilia and increased incidence of bronchus-associated lymphoid tissue. The data from the WT mice provides a global look at mRNA post-natal developmental changes, while the data from the Scnn1b-transgenic line allows differential gene expression due to airway surface liquid dehydration and mucus obstruction to be queried.
The data presented for the lung is part of a larger body of work evaluating gene expression in lung (left lobe only), trachea, and purified macrophages (from bronchoalveolar lavage fluid).
|
| |
|
| Overall design |
24 Total lung (left lobe only) samples were analyzed; three from each timepoint for each genotype (wild type and Scnn1b-transgenic). In our manuscript, we were most interested in changes between WT and Scnn1b-Tg mice, however, the data can also be used to evaluate changes in gene expression across time (PND 0, 3, 10, and 42). We generated the following pairwise comparisons: Scnn1b-Tg vs WT mice for each PND time point; Intra-strain comparison between PND 3, or 10, or 42 vs PND 0.
|
| |
|
| Contributor(s) |
Saini Y, Dang H, Livraghi-Butrico A, O'Neal WK, Boucher RC |
| Citation(s) |
25204199 |
| |
| Submission date |
May 31, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Hong Dang |
| E-mail(s) |
[email protected]
|
| Organization name |
UNC Chapel Hill
|
| Department |
CF Center
|
| Street address |
7027 Thurston-Bowles Bldg. CB#7248
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (24)
|
| GSM1152160 |
Whole lung WT, PND 0, biological replicate 1 |
| GSM1152161 |
Whole lung WT, PND 0, biological replicate 2 |
| GSM1152162 |
Whole lung WT, PND 0, biological replicate 3 |
| GSM1152163 |
Whole lung WT, PND 3, biological replicate 1 |
| GSM1152164 |
Whole lung WT, PND 3, biological replicate 2 |
| GSM1152165 |
Whole lung WT, PND 3, biological replicate 3 |
| GSM1152166 |
Whole lung WT, PND 10, biological replicate 1 |
| GSM1152167 |
Whole lung WT, PND 10, biological replicate 2 |
| GSM1152168 |
Whole lung WT, PND 10, biological replicate 3 |
| GSM1152169 |
Whole lung WT, PND 42, biological replicate 1 |
| GSM1152170 |
Whole lung WT, PND 42, biological replicate 2 |
| GSM1152171 |
Whole lung WT, PND 42, biological replicate 3 |
| GSM1152172 |
Whole lung Scnn1b-Tg, PND 0, biological replicate 1 |
| GSM1152173 |
Whole lung Scnn1b-Tg, PND 0, biological replicate 2 |
| GSM1152174 |
Whole lung Scnn1b-Tg, PND 0, biological replicate 3 |
| GSM1152175 |
Whole lung Scnn1b-Tg, PND 3, biological replicate 1 |
| GSM1152176 |
Whole lung Scnn1b-Tg, PND 3, biological replicate 2 |
| GSM1152177 |
Whole lung Scnn1b-Tg, PND 3, biological replicate 3 |
| GSM1152178 |
Whole lung Scnn1b-Tg, PND 10, biological replicate 1 |
| GSM1152179 |
Whole lung Scnn1b-Tg, PND 10, biological replicate 2 |
| GSM1152180 |
Whole lung Scnn1b-Tg, PND 10, biological replicate 3 |
| GSM1152181 |
Whole lung Scnn1b-Tg, PND 42, biological replicate 1 |
| GSM1152182 |
Whole lung Scnn1b-Tg, PND 42, biological replicate 2 |
| GSM1152183 |
Whole lung Scnn1b-Tg, PND 42, biological replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE47551 |
mRNA expression data from either wild-type mice or Scnn1b-transgenic mice at post-natal days 0, 3, 10, and 42 |
|
| Relations |
| BioProject |
PRJNA206056 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47546_RAW.tar |
92.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...