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Status |
Public on Mar 31, 2014 |
Title |
Expression analysis of OSK and Sall4+OSK reprogramming cells sorted for expression of miR-290 and/or miR-302. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
MEFs were infected with Oct4, Sox2, Klf4 (+/- Sall4), sorted for miR-290/302 reporter expression at day 9 (OSK) or 12 (Sall4+OSK), and then profiled. Resulting iPSCs were also profiled. Mouse ES cells were differentiated and sorted for miR-290/302 reporter expression during Fgf/Activin differentiation (day 4 or day 7).
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Overall design |
Performed in biological triplicate. Biological triplicate samples represent three independent lines. Total RNA collected with Trizol. All processing conducted at the UCLA Neuroscience Genomics Core. MouseRef-8 v2.0 Expression BeadChips.
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Contributor(s) |
Parchem R, Blelloch R |
Citation missing |
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Submission date |
Jan 23, 2014 |
Last update date |
Jun 14, 2018 |
Contact name |
Ron Parchem |
Organization name |
UCSF
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Street address |
35 Medical Center Way
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City |
San Francisco |
ZIP/Postal code |
94143 |
Country |
USA |
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Platforms (1) |
GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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Samples (48)
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Relations |
BioProject |
PRJNA236236 |