|
| Status |
Public on Mar 31, 2014 |
| Title |
OSK-black-2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse reprogramming cells
|
| Organism |
Mus musculus |
| Characteristics |
plate: 1
strain: B6
transfection: OSK
cell type: embryonic fibroblasts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN miRNeasy micro kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
Sample02.AVG_Signal
|
| Data processing |
The data were preprocessed using Combat and quantile normalized in R
|
| |
|
| Submission date |
Jan 23, 2014 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Ron Parchem |
| Organization name |
UCSF
|
| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE54341 |
Expression analysis of OSK and Sall4+OSK reprogramming cells sorted for expression of miR-290 and/or miR-302. |
|
