|
| Status |
Public on Mar 18, 2015 |
| Title |
The expression pattern of microRNAs in granulosa cells of subordinate and dominant follicles during the early luteal phase of bovine estrous cycle |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
This study was conducted to investigate the miRNA enrichment and degradation in the granulosa cells of dominant and subordinate follicle during the early luteal phase of the bovine estrous cycle using high throughput miRNA sequencing technology.
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| Overall design |
We used follicle samples retrieved from ovaries obtained from Simmental heifers slaughtered at day 3 and 7 of the estrous cycle. The follicles were categorized as subordinate (SF) and dominant (DF) based on their size. The granulosa cells were collected from each follicle and subjected to total RNA isolation .The RNA samples used for miRNAs deep sequencing. A total of 12 granulosa samples (three biological replicates of granulosa cells from SF or DF at day 3 or day 7 of the estrous cycle) were used for miRNA deep sequencing.
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| Contributor(s) |
Salilew-Wondim D, Ijaz A, Sudeep S, Gebremedhn S, Rings F, Hoelker M, Tholen E, Christiane N, Looft C, Schellander K, Tesfaye D |
| Citation(s) |
25192015 |
| |
| Submission date |
Mar 18, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Dessie Salilew-Wondim |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Bonn
|
| Department |
Animal breeding and husbandry group
|
| Street address |
Endenicher Allee 15
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
D-53115 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL15749 |
Illumina HiSeq 2000 (Bos taurus) |
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| Samples (12)
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| GSM1349872 |
NG-6435_A26258_S1_lib18493_1362_3_1.fastq.bz2 Biological replicate 1 |
| GSM1349873 |
NG-6435_A26259_S2_lib18494_1362_3_1.fastq.bz2 Biological replicate 2 |
| GSM1349874 |
NG-6435_A26260_S3_lib18495_1362_3_1.fastq.bz2 Biological replicate 3 |
| GSM1349875 |
NG-6435_A26261_S4_lib18496_1362_3_1.fastq.bz2 Biological replicate1 |
| GSM1349876 |
NG-6435_A26262_S5_lib18497_1362_3_1.fastq.bz2 Biological replicate 2 |
| GSM1349877 |
NG-6435_A26263_S6_lib18498_1362_3_1.fastq.bz2 Biological replicate 3 |
| GSM1349878 |
NG-6435_A26267_L1_lib18502_1362_3_1.fastq.bz2 Biological replicate 1 |
| GSM1349879 |
NG-6435_A26268_L2_lib18503_1362_3_1.fastq.bz2 Biological replicate 2 |
| GSM1349880 |
NG-6435_A26269_L3_lib18504_1362_3_1.fastq.bz2 Biological replicate 3 |
| GSM1349881 |
NG-6435_A26270_L4_lib18505_1362_3_1.fastq.bz2 Biological replicate 1 |
| GSM1349882 |
NG-6435_A26271_L5_lib18506_1362_3_1.fastq.bz2 Biological replicate 2 |
| GSM1349883 |
NG-6435_A26272_L6_lib18507_1362_3_1.fastq.bz2 Biological replicate 3 |
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| Relations |
| BioProject |
PRJNA241945 |
| SRA |
SRP040270 |
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