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| Status |
Public on Sep 09, 2014 |
| Title |
Contrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes. |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
Poly-ribosomal mRNA from pools of 75 bovine oocytes at the germinal vesicle(GV) and metaphase II(MII) stages were isolated by sucrose fractionation then compared through microarray hybridization.
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| Overall design |
3 biological replicates from germinal vesicle(GV) and metaphase II(MII) bovine oocytes were hybridized against each other while alternating dyes. One additional technical replicate (dye-swap of one of the previous hybridizations) was added to balance dye usage.
The normalized log2 ratio (GV/MII) data is provided in the 'normalized_data.txt' file. Please note that data were scanned/extracted using a different Agilent reference file, and thus the feature extraction number for a given probe (in both normalized and raw data files) is different from that in the official Agilent-041017 platform (GPL18537). The reference file used for this study is provided as a Series supplementary file (041017_D_DNAFront_BCBottom_20120517.txt).
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| Contributor(s) |
Scantland S, Tessaro I, Macabelli CH, Cagnone G, Fournier E, Macaulay A, Luciano AM, Robert C |
| Citation(s) |
25078684, 26586844 |
| |
| Submission date |
Apr 08, 2014 |
| Last update date |
Feb 05, 2016 |
| Contact name |
Eric Fournier |
| E-mail(s) |
[email protected]
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| Organization name |
Universite Laval
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| Department |
Departement des sciences animales
|
| Street address |
2325 Rue de l'Universite
|
| City |
Quebec |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
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| Platforms (1) |
| GPL18537 |
Agilent-041017 Embryogene Bovine 45K microarray with drosophila controls v.2.1 [EMBV_Droso_V2.1] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA244050 |
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