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Series GSE6237

Query DataSets for GSE6237

Status

Public on Aug 05, 2008

Title

Fine temporal analysis of DHT transcriptional modulation of the ATM/Gadd45g signaling pathways in the mouse uterus.

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

In rodents, the uterus of a mature
female undergoes changes during the uterine cycle,
under the control of steroid hormones. 5a-Dihydrotestosterone
(DHT) is recognized to play an important
role in the regulation of androgen action in normal
endometrium. Using microarray technology, a screening
analysis of genes responding to DHT in the uterus
of ovariectomized mice, has allowed us to highlight
multiple genes of the ATM/Gadd45g pathway that
are modulated following exposure to DHT. Two phases
of regulation were identified. In the early phase, the
expression of genes involved in the G2/M arrest is
rapidly increased, followed by the repression of genes
of the G1/S checkpoint, and by the induction of
transcriptional regulators. Later, i.e. from 12 to 24 hr,
genes involved in G2/M transition, cytoarchitectural
and lipid-related genes are stimulated by DHT while
immunity-related genes appear to be differentially
regulated by the hormone. These results show that a
physiological dose of DHT induces the transcription of
genes promoting the cell cycle progression in mice.
Profile determination of temporal uterine gene expression
at the transcriptional level enables us to suggest
that the DHT modulation of genes involved in ATM/
Gadd45g signaling in an ATM- or p53-independent
manner, could play an important role in the cyclical
changes of uterine cells in the mouse uterus.
Keywords: DHT time course

Overall design

Ten 10-11 weeks old C57Bl/6 mice were gonadectomized under isoflurane-induced anesthesia and sacrified seven days after GDX. Mice were injected s.c with DHT (0.1mg/mice) or vehicle, at 0.5, 1, 3, 6, 12 or 24 h before sacrifice. Uteri was removed and pooled before total RNA extraction. Samples was hybridized to U74Av2 Genechips (Affymetrix).

Contributor(s)

Durocher F, Ivanga M, Calvo E, Labrie Y, Labrie F

Citation(s)

18671277

Submission date

Nov 07, 2006

Last update date

Feb 18, 2018

Contact name

Ezequiel L Calvo

E-mail(s)

[email protected]