|
| Status |
Public on Jul 11, 2015 |
| Title |
Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-TAB-seq |
| Organisms |
synthetic construct; Caldicellulosiruptor acetigenus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
N4-methylcytosine is a major DNA modification integral to restriction-modification (R-M) systems in bacterial genomes. Here we describe 4mC-Tet-Assisted Bisulfite-sequencing (4mC-TAB-seq), a method that accurately and rapidly reveals the genome-wide locations of N4-methylcytosines at single-base resolution. By coupling Tet-mediated oxidation with a modified sodium bisulfite conversion reaction, unmodified cytosines and 5-methylcytosines are read out as thymines, whereas N4-methylcytosines are read out as cytosines revealing their positions throughout the genome.
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| |
|
| Overall design |
4mC-TAB-seq
|
| |
|
| Contributor(s) |
Schmitz RJ |
| Citation(s) |
26184871 |
| |
| Submission date |
Nov 17, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19423 |
Illumina NextSeq 500 (Caldicellulosiruptor kristjanssonii) |
| GPL19424 |
Illumina NextSeq 500 (synthetic construct) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA267586 |
| SRA |
SRP049960 |
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