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Sample GSM1547609

Query DataSets for GSM1547609

Status

Public on Jul 11, 2015

Title

76 mer control 25%

Sample type

SRA

Source name

pUC19 vector DNA

Organism

synthetic construct

Characteristics

tissue: pUC19 vector DNA

Treatment protocol

N/A

Growth protocol

Caldicellulosiruptor kristjanssonii wild-type strain was grown anaerobically in liquid low osmolarity complex growth (LOC) medium17 (final pH 7.0) with maltose (0.5% w/v; Sigma M5895) as the carbon source.

Extracted molecule

genomic DNA

Extraction protocol

Genomic DNA (gDNA) was purified from Arabidopsis thalina leaf tissue using a Qiagen DNease Plant Kit (Qiagen, Valencia, CA), according to the manufacturer’s instructions. MethylC-Seq libraries were constructed according to Urich MA, Nery JR, Lister R, Schmitz RJ, Ecker JR. (2014) MethylC-seq: Base resolution whole genome bisulfite sequencing library preparation. Nature Protocols (in press)

Library strategy

Bisulfite-Seq

Library source

genomic

Library selection

RANDOM

Instrument model

Illumina NextSeq 500

Data processing

Data were mapped to the Caldicellulosiruptor kristjanssonii wild type reference genome
MethylC-seq analysis were performed as described in Schmtiz et al. Epigenome-wide inheritance of DNA methylation variants in a recombinant inbred population. Genome Research (2013).
Genome_build: Caldicellulosiruptor kristjanssonii wild type reference genome
Supplementary_files_format_and_content: tab delimited text file

Submission date

Nov 17, 2014

Last update date

May 15, 2019

Contact name

Robert J Schmitz

E-mail(s)

[email protected]

Organization name

University of Georgia

Department

Genetics