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| Status |
Public on Jun 13, 2017 |
| Title |
Rice Gene Network Inferred from Expression Profiling of Plants Carrying Xa3/Xa26. |
| Platform organism |
Oryza sativa |
| Sample organism |
Oryza sativa Japonica Group |
| Experiment type |
Expression profiling by array
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| Summary |
Japonica rice (Oryza sativa ssp. japonica) variety Mudanjiang 8 (MDJ8) is the wild-type and is susceptible to Xoo. Transgenic rice line Rb49 carries the MR gene Xa3/Xa26, which is driven by its native promoter with the genetic background of MDJ8, and this line is resistant to certain strains (including strain PXO61) of Xoo. Although many studies on Xa3/Xa26-mediated resistace to rice Xoo have been published, the molecular mechanism of this major resistance gene remains poorly understood. Here, we use affymetrix microarray technology to analyze the regulated network mediated by Xa3/Xa26
We used microarrays to study the gene expression network mediated by Xa3/Xa26. Plants were inoculated with the Xoo strain PXO61 at the four-leaf to five-leaf stage by the leaf-clipping method. Control rice plants were inoculated with water (mock inoculation). Samples were collected before inoculation (ck) and at 2, 4, and 24 hours after PXO61 or mock inoculation from Rb49 and MDJ8. Leaf fragments approximately 2 cm in length that were immediately next to the inoculation site were collected.
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| Overall design |
Rice leaves collected at different time points post mock or PXO61 treatments were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the regulated network mediated by Xa3/Xa26.
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| Contributor(s) |
Hong H, Liu Y, Zhang H, Xiao J, Li X, Wang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 26, 2015 |
| Last update date |
Dec 27, 2017 |
| Contact name |
hanming hong |
| Organization name |
NCPGR
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| Street address |
SHIZHISHAN
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| City |
WUHAN |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (28)
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| GSM1695752 |
MDJ8 before treatment, biological rep1 |
| GSM1695753 |
MDJ8 before treatment, biological rep2 |
| GSM1695754 |
MDJ8 with 2 hours post mock treatment, biological rep1 |
| GSM1695755 |
MDJ8 with 2 hours post mock treatment, biological rep2 |
| GSM1695756 |
MDJ8 with 4 hours post mock treatment, biological rep1 |
| GSM1695757 |
MDJ8 with 4 hours post mock treatment, biological rep2 |
| GSM1695758 |
MDJ8 with 24 hours post mock treatment, biological rep1 |
| GSM1695759 |
MDJ8 with 24 hours post mock treatment, biological rep2 |
| GSM1695760 |
MDJ8 with 2 hours post PXO61 treatment, biological rep1 |
| GSM1695761 |
MDJ8 with 2 hours post PXO61 treatment, biological rep2 |
| GSM1695762 |
MDJ8 with 4 hours post PXO61 treatment, biological rep1 |
| GSM1695763 |
MDJ8 with 4 hours post PXO61 treatment, biological rep2 |
| GSM1695764 |
MDJ8 with 24 hours post PXO61 treatment, biological rep1 |
| GSM1695765 |
MDJ8 with 24 hours post PXO61 treatment, biological rep2 |
| GSM1695766 |
Rb49 before treatment, biological rep1 |
| GSM1695767 |
Rb49 before treatment, biological rep2 |
| GSM1695768 |
Rb49 with 2 hours post mock treatment, biological rep1 |
| GSM1695769 |
Rb49 with 2 hours post mock treatment, biological rep2 |
| GSM1695770 |
Rb49 with 4 hours post mock treatment, biological rep1 |
| GSM1695771 |
Rb49 with 4 hours post mock treatment, biological rep2 |
| GSM1695772 |
Rb49 with 24 hours post mock treatment, biological rep1 |
| GSM1695773 |
Rb49 with 24 hours post mock treatment, biological rep2 |
| GSM1695774 |
Rb49 with 2 hours post PXO61 treatment, biological rep1 |
| GSM1695775 |
Rb49 with 2 hours post PXO61 treatment, biological rep2 |
| GSM1695776 |
Rb49 with 4 hours post PXO61 treatment, biological rep1 |
| GSM1695777 |
Rb49 with 4 hours post PXO61 treatment, biological rep2 |
| GSM1695778 |
Rb49 with 24 hours post PXO61 treatment, biological rep1 |
| GSM1695779 |
Rb49 with 24 hours post PXO61 treatment, biological rep2 |
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| Relations |
| BioProject |
PRJNA284942 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69235_RAW.tar |
124.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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