|
| Status |
Public on Jun 13, 2017 |
| Title |
MDJ8 with 2 hours post mock treatment, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MDJ8 leaf with 2 hours post mock treatment
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
tissue: leaf (four-leaf to five-leaf stage)
genotype/variation: MDJ8
treatment: 2 hours post mock treatment
|
| Treatment protocol |
Plants were inoculated with the Xoo strain PXO61 at the four-leaf to five-leaf stage by the leaf-clipping method. Control rice plants were inoculated with water (mock inoculation).
|
| Growth protocol |
Rice plants were grown in the field under nonstressed conditions during the rice-growing season in Wuhan, Hubei provice, China.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Oryza sativa Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from mudanjiang8(MDJ8) with 2 hours post mock treatment
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 26, 2015 |
| Last update date |
Jun 13, 2017 |
| Contact name |
hanming hong |
| Organization name |
NCPGR
|
| Street address |
SHIZHISHAN
|
| City |
WUHAN |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE69235 |
Rice Gene Network Inferred from Expression Profiling of Plants Carrying Xa3/Xa26. |
|
