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| Status |
Public on Jun 30, 2007 |
| Title |
Expression data from Mus musculus Myc-null heart at E13.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We created mice, which are deficient for Myc specifically in cardiac myocytes by crossing crossed Myc-floxed mice (Mycfl/fl) and MLC-2VCre/+ mice. Serial analysis of earlier stages of gestation revealed that Myc-deficient mice died prematurely at E13.5-14.5. Morphological analyses of E13.5 Myc-null embryos showed normal ventricular size and structure; however, decreased cardiac myocyte proliferation and increased apoptosis was observed. BrdU incorporation rates were also decreased significantly in Myc-null myocardium. Myc-null mice displayed a 3.67-fold increase in apoptotic cardiomyocytes by TUNEL assay.
We examined global gene expression using oligonucleotide microarrays. Numerous genes involved in mitochondrial death pathways were dysregulated including Bnip3L and Birc2.
Keywords: wildtype vs Myc-null
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| Overall design |
Hearts were taken from wide type and Myc-null Mouse embryos at E13.5 under the dissecting scope. Cardiac myocyte RNA was isolated using TRIZOL®Reagent Total RNA (100 ng) was hybridized to the Sentrix® MouseRef-8 Expression BeadChip that contains probes for ~24,000 transcripts. GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A. The data were analyzed with Illumina Inc. BeadStudio version 1.5.0.34 and normalized by rank invariant method.
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| Web link |
http://www.mednet.ucla.edu
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| |
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| Contributor(s) |
Zhong W, Angelis E, Schenke-Layland K, Gong W, MacLellan RW |
| Citation missing |
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| |
| Submission date |
Feb 28, 2007 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Weiguang Zhong |
| E-mail(s) |
[email protected]
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| Organization name |
UCLA
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| Department |
Cardiology
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| Street address |
675 Charles Young Drive
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL4865 |
Sentrix MouseRef-8 Expression BeadChip (Target ID) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA98167 |
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