|
| Status |
Public on Jun 30, 2007 |
| Title |
Embryo heart at E13.5, KO-4 |
| Sample type |
RNA |
| |
|
| Source name |
KO Mouse embryo hearts between E12.5-15.5
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: wildtype vs Myc-null
Cardiac myocyte proliferation and apoptosis comparison, effect of wildtype vs Myc-null hearts
|
| Treatment protocol |
Embryos and tissues were taken from various stages under the dissecting scope. Selected hearts were rinsed with ice cool PBS and placed in the Trizol solution (GibcoBRL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cardiac myocyte RNA was isolated using TRIZOL®Reagent (Sigma). RNA quality was assessed using an Agilent 2100 bioanalyzer.
|
| Label |
Biotin Streptavidin-Cy3
|
| Label protocol |
Biotinylated cRNA were synthesized by the Ambion total prep RNA amplification kit. cDNA was quantified and normalized to 77 ng/mcL
|
| |
|
| Hybridization protocol |
Total RNA (100 ng) was hybridized to the Sentrix® MouseRef-8 Expression BeadChip (Illumina, Inc. San Diego, CA) that contains probes for ~20,000 transcripts
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Data processing |
The data were analyzed with Illumina Inc. BeadStudio version 1.5.0.34 and normalized by rank invariant method.
|
| |
|
| Submission date |
Feb 28, 2007 |
| Last update date |
Jun 29, 2007 |
| Contact name |
Weiguang Zhong |
| E-mail(s) |
[email protected]
|
| Organization name |
UCLA
|
| Department |
Cardiology
|
| Street address |
675 Charles Young Drive
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL4865 |
| Series (1) |
| GSE7162 |
Expression data from Mus musculus Myc-null heart at E13.5 |
|
