|
| Status |
Public on Feb 18, 2016 |
| Title |
Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activity in the recruitment of Smc/ScpAB to the Bacillus subtilis chromosome. We demonstrate that targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. We find that dimerization of the Smc hinge domain stabilizes closed Smc rods and hinders head engagement as well as chromosomal targeting. Conversely, the ScpAB sub-complex promotes head engagement and Smc rod opening and thereby facilitates recruitment of Smc to parS sites. Upon ATP hydrolysis, Smc/ScpAB is released from loading sites and relocates within the chromosome—presumably through translocation along DNA double helices. Our findings define an intermediate state in the process of chromosome organization by Smc.
|
| |
|
| Overall design |
ChIP-Seq experiments were performed on wild type and mutant cells of Bacillus subtilis 1A700.
|
| |
|
| Contributor(s) |
Minnen A, Bürmann F, Wilhelm L, Anchimiuk A, Diebold-Durand M, Gruber S |
| Citation(s) |
26904953 |
| |
| Submission date |
Jan 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephan Gruber |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute of Biochemistry
|
| Street address |
Am Klopferspitz 18
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL21344 |
Illumina HiSeq 2500 (Bacillus subtilis subsp. subtilis str. 168) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA309121 |
| SRA |
SRP068568 |
Less...
More...