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| Status |
Public on Aug 01, 2016 |
| Title |
Scots pine (Pinus sylvestris) differentially interacts with Cenococcum geophilum and other simultaneously inoculated mycorrhizal (ECM) fungi |
| Platform organisms |
Pinus sylvestris; Cenococcum geophilum |
| Sample organism |
Pinus sylvestris |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify specific gene networks induced in host roots by C. geophilum, we inoculated seedlings of Scots pine simultaneously with C. geophilum and either Suillus granulatus or Rhizopogon roseolus, two common ECM fungi associated to pines. We then measured the differential expression of Scots pine genes in the respective mycorrhizas using oligoarrays.
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| Overall design |
We performed 14 hybridizations (NimbleGen) with samples derived from Pinus sylvestris mycorrhiza with Cenococcum geophilum, Rhizopogon roseolus or Suillus granulatus (3 biological replicates each), as well as from non-mycorrhizal control roots (two replicates). Only the Pinus-derived sequences from the array were considered for this analysis. All samples were labeled with Cy3.
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| Contributor(s) |
Peter M, Tisserant E, Kohler A, Martin F |
| Citation(s) |
27601008 |
| |
| Submission date |
Apr 21, 2016 |
| Last update date |
Nov 02, 2016 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
[email protected]
|
| Phone |
+33 (0)383 394072
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| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
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| Platforms (1) |
| GPL21757 |
NimbleGen Cenococcum geophilum-Pinus sylvestris mixed 12-plex expression array [100616_CG-Pinus_EXP] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA319254 |
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