|
| Status |
Public on Aug 01, 2016 |
| Title |
CR_Rhizopogon_1 |
| Sample type |
RNA |
| |
|
| Source name |
Pinus inoculated with Cenococcum/Rhizopogon, Rhizopogon-Pinus mycorrhiza
|
| Organism |
Pinus sylvestris |
| Characteristics |
inoculated fungi: Cenococcum geophilum, Rhizopogon roseolus
tissue: Rhizopogon-Pinus mycorrhiza
|
| Growth protocol |
Double-mycorrhized P. sylvestris seedlings were produced using C. geophilum 1.58 and the abundant pine symbiotic ECM species Rhizopogon roseolus (Corda) Th. Fr. or Suillus granulatus (L.) Roussel. Four months after inoculation, three and two replicate plants were harvested for each fungal combination and for non-mycorrhizal plants, respectively. Single fine root tips were collected for each ECM species per plant and for non-mycorrhizal plants in separate tubes and were shock-frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the NucleoSpin RNA Plant kit (MACHEREY-NAGEL) or the Power Plant RNA isolation kit (MOBIO), and cDNA was amplified using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company.
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company.
|
| Scan protocol |
Scanning by NimbleGen Company.
|
| Description |
R_CR1_55941802
Rhizopogon mycorrhiza from Pinus sylvestris, inoculated with both Cenococcum and Rhizopogon.
It is the first of three biological replicates used in this experiment.
|
| Data processing |
Procedure for spot detection was performed by NimbleGen Company. Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA). For information on RMA, see Biostatistics 2003 Apr; 4(2): 249-264.
|
| |
|
| Submission date |
Apr 21, 2016 |
| Last update date |
Aug 01, 2016 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
[email protected]
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL21757 |
| Series (1) |
| GSE80549 |
Scots pine (Pinus sylvestris) differentially interacts with Cenococcum geophilum and other simultaneously inoculated mycorrhizal (ECM) fungi |
|
