|
| Status |
Public on Jun 20, 2017 |
| Title |
Identification of Amino Enhancer of Split (AES) and DDX21 binding snoRNAs by RIP-Seq. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We studied AES and DDX21 binding RNAs in Kasumi-1 cells stably expressing V5-tagged AES. RNA immunoprecipitation was performed with V5 antibody (for AES), DDX21 antibody and control IgG. We found that AES as well as DDX21 RIP samples showed enrichment for small nucleolar RNAs (snoRNAs) compared to control IgG. We also showed that AES and DDX21 binding snoRNAs showed significant overlap. Our studies provide mechanisms how AES regulates snoRNAs and rRNA modification.
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| |
|
| Overall design |
RIP followed by small RNA sequencing in Kasumi-1 cells
|
| |
|
| Contributor(s) |
Rohde C, Zhou F, Bartenhagen C, Müller-Tidow C |
| Citation(s) |
28650479 |
| |
| Submission date |
Apr 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christian Rohde |
| E-mail(s) |
[email protected]
|
| Organization name |
Heidelberg University
|
| Lab |
Molecular Hematology and Oncology
|
| Street address |
Im Neuenheimer Feld 410
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE80602 |
AML1-ETO induces leukemia via C/D box snoRNA/RNPs. |
|
| Relations |
| BioProject |
PRJNA319362 |
| SRA |
SRP073719 |
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