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Status |
Public on Apr 28, 2016 |
Title |
Expression data from whole Drosophila pupa of 16-17 hr APF stage |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by array
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Summary |
Expression of activated Ras along with alteration in levels of non-coding RNA hsromega in the eye disc results in lowered ecdysone levels in post pupation stages, resulting in early pupal death. This microarray analysis of total pupal RNAs was undertaken to know how could expression of activated Ras with altered levels of hsromega RNA in eye discs could reduce ecdysone levels
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Overall design |
16-17 hr old pupae expressing activated Ras under sevGAL4 either alone or in background of altered levels of hsromega non coding RNA were selected for RNA extraction and hybridization on Affymetrix microarrays. Pupae of same stage of individuals expressing only UAS-GFP under the same driver were used as control
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Contributor(s) |
Ray M, Lakhotia SC |
Citation |
Mukulika Ray, Subhash C. Lakhotia. Activated Ras expression in eye discs with altered hsrw lncRNA causes JNK-induced Dilp8 secretion and reduces post-pupal ecdysone leading to early pupal death in Drosophila. BiorXiv. doi: http://dx.doi.org/10.1101/049882
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Submission date |
Apr 26, 2016 |
Last update date |
May 04, 2018 |
Contact name |
Subhash C Lakhotia |
E-mail(s) |
[email protected]
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Phone |
+91-542-2368145
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Organization name |
Banaras Hindu UNiversity
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Department |
Zoology
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Street address |
Cytogenetics Lab
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City |
Varanasi |
State/province |
UP |
ZIP/Postal code |
221005 |
Country |
India |
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Platforms (1) |
GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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Samples (8)
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Relations |
BioProject |
PRJNA319743 |