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Series GSE80703 Query DataSets for GSE80703
Status Public on Apr 28, 2016
Title Expression data from whole Drosophila pupa of 16-17 hr APF stage
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Expression of activated Ras along with alteration in levels of non-coding RNA hsromega in the eye disc results in lowered ecdysone levels in post pupation stages, resulting in early pupal death.
This microarray analysis of total pupal RNAs was undertaken to know how could expression of activated Ras with altered levels of hsromega RNA in eye discs could reduce ecdysone levels
 
Overall design 16-17 hr old pupae expressing activated Ras under sevGAL4 either alone or in background of altered levels of hsromega non coding RNA were selected for RNA extraction and hybridization on Affymetrix microarrays. Pupae of same stage of individuals expressing only UAS-GFP under the same driver were used as control
 
Contributor(s) Ray M, Lakhotia SC
Citation Mukulika Ray, Subhash C. Lakhotia. Activated Ras expression in eye discs with altered hsrw lncRNA causes JNK-induced Dilp8 secretion and reduces post-pupal ecdysone leading to early pupal death in Drosophila. BiorXiv. doi: http://dx.doi.org/10.1101/049882
Submission date Apr 26, 2016
Last update date May 04, 2018
Contact name Subhash C Lakhotia
E-mail(s) [email protected]
Phone +91-542-2368145
Organization name Banaras Hindu UNiversity
Department Zoology
Street address Cytogenetics Lab
City Varanasi
State/province UP
ZIP/Postal code 221005
Country India
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (8)
GSM2133777 sev-GAL4>UAS-GFP_1
GSM2133778 sev-GAL4>UAS-GFP_2
GSM2133779 sev-GAL4>Ras1V12_1
Relations
BioProject PRJNA319743

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80703_RAW.tar 15.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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