|
| Status |
Public on Apr 28, 2016 |
| Title |
sev-GAL4>Ras1V12_2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila melanogaster 16-17 hr old pupae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Pupa without pupal case
genotype: sev-GAL4>Ras1V12
age: 16-17 hr APF
|
| Treatment protocol |
16-17 hr APF sev-GAL4>UAS-GFP, sev-GAL4>Ras1V12, sev-GAL4>Ras1V12 hsrω-RNAi and sev-GAL4>Ras1V12 EP3037 pupae were dissected in 1X PBS to remove the pupal case while all the internal tissues were placed on ice in the TriReagent solution (Sigma Aldrich) for RNA extraction.
|
| Growth protocol |
0-1 hr APF stage of sev-GAL4>UAS-GFP, sev-GAL4>Ras1V12, sev-GAL4>Ras1V12 hsrω-RNAi and sev-GAL4>Ras1V12 EP3037 pupae were collected and kept at 24°C and allowed to age for 16-17 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriReagent extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 500 ng of total RNA (Affymetrix GeneChip Expression Analysis Technical Manual using the GeneChip 3’ IVT Plus Reagent Kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 µg of cRNA samples were hybridized for 16 hr at 45°C on Drosophila Genome 2.0 microarray chips for 3’ IVT array . GeneChips were washed and stained in the Affymetrix GeneChip® Fluidics station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000
|
| Description |
Gene Expression data from 16-17 hr APF whole pupa expressing activated Ras protein in eye disc under sev-GAL4
|
| Data processing |
Summary of the expression levels for each gene in the four genotypes was obtained from the Expression Console software and Affymetrix Transcription analysis console.
|
| |
|
| Submission date |
Apr 26, 2016 |
| Last update date |
Apr 28, 2016 |
| Contact name |
Subhash C Lakhotia |
| E-mail(s) |
[email protected]
|
| Phone |
+91-542-2368145
|
| Organization name |
Banaras Hindu UNiversity
|
| Department |
Zoology
|
| Street address |
Cytogenetics Lab
|
| City |
Varanasi |
| State/province |
UP |
| ZIP/Postal code |
221005 |
| Country |
India |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE80703 |
Expression data from whole Drosophila pupa of 16-17 hr APF stage |
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