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| Status |
Public on Jun 14, 2007 |
| Title |
Pooh cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
POU5F1 (more commonly known as Oct-4/3) is one of the stem cell markers and affects direction of differentiation in embryonic stem cells. To investigate whether cells of mesenchymal origin acquire embryonic phenotype, we generated a human cell line of mesodermal origin with overexpression of the chimeric POU5F1 gene with physiological co-activator EWS, which is driven by the potent EWS promoter by translocation. The cell line termed Pooh (POU5F1/Oct-4/3 overexpressing human) cells expressed embryonic stem cell genes such as Nanog and also non-translocated POU5F1, lost mesenchymal phenotypes, and exhibited embryonal stem cell-like alveolar structure when implanted into the subcutaneous tissue of immunodeficient mice. Hierarchical analysis by microchip analysis and cell surface analysis revealed that Pooh cells are subcategorized into the group of human embryonic stem cells and embryonal carcinoma cells. These results imply that cells of mesenchymal origin can partially be traced back to cells to embryonic phenotype by the POU5F1 gene in collaboration with the potent cis-regulatory element and the fused co-activator.
Keywords: Involvement of chimeric POU5F1 (Oct-4/3) with physiological co-activator EWS in mesenchymal-embryonic transition of human cells
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| Overall design |
Pooh cells was generated from primary or first passage cells of pelvic tumor (Yamaguchi et al., Genes Chromosomes Cancer. 2005 Jun;43(2):217-22), and cultured in tissue culture dishes (100 mm, Becton Dickinson) in the G031101 medium (Med Shirotori, Tokyo). All cultures were maintained at 37°C in a humidified atmosphere containing 95% air and 5% CO2.
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| Contributor(s) |
Makino H, Umezawa A |
| Citation(s) |
19559696 |
| |
| Submission date |
Jun 13, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
[email protected]
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| Phone |
81-3-5494-7047
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| Organization name |
National Center for Child Health and Development
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| Department |
Center for Regenerative Medicine
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| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (9)
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| GSM201137 |
POU5F1/Oct-4/3 overexpressing human cell |
| GSM201138 |
5-azacytidine-treatd POU5F1/Oct-4/3 overexpressing human cell |
| GSM201139 |
Human yolk sac tumor |
| GSM201140 |
Complex type germ cell tumor of human embryonic testis origin (1754NCR-G2Ad) |
| GSM201141 |
Complex type germ cell tumor of human embryonic testis origin (1299NCR-G3NAd) |
| GSM201142 |
Complex type germ cell tumor of human embryonic testis origin (1756NCR-G4Ad) |
| GSM201143 |
Bone marrow stromal cell (1112H4-1) |
| GSM201144 |
Bone marrow stromal cell (1838Yub610_10F) |
| GSM201145 |
Bone marrow stromal cell (1773BMSC3F0664) |
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| Relations |
| BioProject |
PRJNA100983 |
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