|
| Status |
Public on Jun 14, 2007 |
| Title |
POU5F1/Oct-4/3 overexpressing human cell |
| Sample type |
RNA |
| |
|
| Source name |
Human cell line established from the pelvic tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Human female undifferentiated bone tumor of the pelvis (1623GST6)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated with the RNeasy Mini Kit (Qiagen), according to manufacture's instructions.). Genomic DNA was eliminated by DNase I (TAKARA BIO INC.) treatments.
|
| Label |
biotin
|
| Label protocol |
cDNA was subjected to in vitro transcription in the presence of biotinylated nucleoside triphosphates using the Enzo BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences), according to the manufacturer's protocol (One-Cycle Target Labeling and Control Reagent package).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized with a probe array for 16h at 45C, and the hybridized biotinylated cRNA was stained with streptavidin-PE.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (Palo Alto).
|
| Description |
POU5F1/Oct-4/3 overexpressing human cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 13, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
[email protected]
|
| Phone |
81-3-5494-7047
|
| Organization name |
National Center for Child Health and Development
|
| Department |
Center for Regenerative Medicine
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
|
