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| Status |
Public on Nov 03, 2016 |
| Title |
Expression data from D.melanogaster guts with a constitutively active Imd in the presence or absence of a microbiome |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Innate immune responses contributed to the containment of intestinal microbes.
We used microarrays to examine transcriptional profiles of imflamed guts raised in convential or germ-free conditions. We found that gut inflammation and the gut microbiome regulate the expression of several hundred host genes.
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| Overall design |
Genotype control flies and flies with a constitutively active Imd were raised on regular food or food supplemented with antibiotics to obtain conventionally reared (CR) or germ-free (GF) flies. Guts were dissected for RNA extraction and RNA was hybridized to Affymetrix GeneChip®Drosophila Genome 2.0 arrays to study the effects of gut inflammation in a microbiome dependant or independant manner on the transcriptional profile in host's gut.
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| Contributor(s) |
Petkau K, Foley E |
| Citation(s) |
28834743 |
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| Submission date |
Nov 02, 2016 |
| Last update date |
May 04, 2018 |
| Contact name |
Edan Foley |
| E-mail(s) |
[email protected]
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| Organization name |
University of Alberta
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| Department |
Medical Microbiology and Immunology
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| Lab |
University of Alberta
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| Street address |
116 Street and 86 Ave
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| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2S3 |
| Country |
Canada |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA352228 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89445_RAW.tar |
24.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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