|
| Status |
Public on Nov 03, 2016 |
| Title |
CR esg[ts] gut, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila CR esg[ts] guts 2 days post activation
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dissected gut
genotype: w; esgGAL4, tubGAL80[ts], UASGFP/+;+/+
|
| Treatment protocol |
After the 2 day heat shift, a total of 10 guts per sample (5 female, 5 male) were dissected into Trizol and used for RNA extraction.
|
| Growth protocol |
Flies were raised on regular food or food containing an antibiotic cocktail for 10-11 days at 18°C to obtain conventionally reared (CR) or germ-free (GF) flies. The flies were then heat shifted to 29°C for 2 days to get ImdCA expression in gut progenitor cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix GeneChip 3' IVT plus protocol from 100 ng total RNA (User Guide GeneChip® 3' IVT PLUS Reagent Kit, 2013-2015, Affymetrix).
|
| |
|
| Hybridization protocol |
12 ug of cRNA was used for fragmentation, fragmented cRNA was hybridized on GeneChip®Drosophila Genome 2.0 Array, washed and stained according to the Affymetrix protocol (User Guide GeneChip® 3' IVT PLUS Reagent Kit, 2013-2015, Affymetrix).
|
| Scan protocol |
Affymetrix GeneChip 3000 7G Scanner
|
| Description |
Gene expression data from CR esg[ts] guts
|
| Data processing |
The data were analyzed with Expression Console (Affymetrix) using default analysis settings.
|
| |
|
| Submission date |
Nov 02, 2016 |
| Last update date |
Nov 03, 2016 |
| Contact name |
Edan Foley |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Alberta
|
| Department |
Medical Microbiology and Immunology
|
| Lab |
University of Alberta
|
| Street address |
116 Street and 86 Ave
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2S3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE89445 |
Expression data from D.melanogaster guts with a constitutively active Imd in the presence or absence of a microbiome |
|
