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| Status |
Public on Jan 10, 2008 |
| Title |
Gene expression of GidA mutant Streptococcus pyogenes |
| Organism |
Streptococcus pyogenes |
| Experiment type |
Expression profiling by array
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| Summary |
In Streptococcus pyogenes, mutation of GidA results in avirulence despite the same growth rate as the wild type. To understand the basis of this effect, global transcription profiling was conducted.
Keywords: Wild type vs. GidA mutant Streptococcus pyogenes
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| Overall design |
A S. pyogenes genespecific microarray, consisting of 60 to 80-mer oligonucleotides representing 1517 genes out of a total of 1752 predicted open reading frames in the SF370 genome, was constructed. S. pyogenes wild type strain (HSC5) or TM-GidA2 strain (GidA mutant derived form the wild type strain HSC5) were grown in Todd-Hewitt broth supplemented with 0.2% yeast extract (THY medium) to mid-logarithmic phase of growth (OD600=0.3). RNA was extracted, amplified, and hybridized to the arrays.
Each experiment consisted of a pair-wise competitive hybridization of amplified RNA samples from the wild type and GidA mutant derived RNA. Since 2 biological replicates were tested in duplicate with a reciprocal dye-flip, a total of eight DNA microarrays were used for comparison for each set of RNA amplifications.
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| Contributor(s) |
Cho K, Crosby SD, Caparon MG |
| Citation(s) |
18426891 |
| |
| Submission date |
Nov 24, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
KyuHong Cho |
| E-mail(s) |
[email protected]
|
| Organization name |
Southern Illinois University Carbondale
|
| Department |
Microbiology
|
| Street address |
1125 Lincoln Dr. Room 131
|
| City |
Carbondale |
| State/province |
IL |
| ZIP/Postal code |
62901 |
| Country |
USA |
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| Platforms (1) |
| GPL4584 |
WUSTL Streptococcus pyogenes 3K |
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| Samples (8)
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| GSM244283 |
Gene expression profile of the GidA mutant-1 |
| GSM244655 |
Gene expression profile of the GidA mutant-2 |
| GSM244656 |
Gene expression profile of the GidA mutant-3 |
| GSM244657 |
Gene expression profile of the GidA mutant-4 |
| GSM244658 |
Gene expression profile of the GidA mutant-5 |
| GSM244659 |
Gene expression profile of the GidA mutant-6 |
| GSM244660 |
Gene expression profile of the GidA mutant-7 |
| GSM244661 |
Gene expression profile of the GidA mutant-8 |
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| Relations |
| BioProject |
PRJNA103569 |
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