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| Status |
Public on Feb 01, 2008 |
| Title |
Wild type vs glnPA double mutant |
| Organism |
Streptococcus pneumoniae |
| Experiment type |
Expression profiling by array
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| Summary |
This microarray experiment is part of a study addressing the importance of glutamine metabolism in Streptococcus pneumoniae for the virulence of this bacterium. To be able to gain more insight into the phenotype of a double mutant of glnA (TIGR 4 locus tag SP0502, encoding glutamine synthetase) and glnP (SP1241, encoding a permease component of a glutmine uptake ABC transporter), the transcriptome of this mutant, the glnPA double mutant, was determined in strain S. pneumoniae D39 grown in rich GM17 medium with 0.5 mg/ml glutamine. This revealed a big change in transcriptome in the mutant, especially a lot of amino acid and peptide metabolic genes.
Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant
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| Overall design |
The wild-type strain D39 and its isogenic glnPA double mutant were grown in 4 biological replicates in GM17 with 0.5 mg/ml glutamine to an Optical Density at 600 nm of 0.3. From these cultures samples were prepared for transciptome determination with in-house printed microarrays covering the genomes of several S. pneumoniae species. The wild-type (control, Ch1) was labeled with Cy5 (3 samples) or Cy3 (1 sample) and vice versa for the samples of the glnPA mutant (target, Ch2). Samples were pair-wise compared on glass-microarray slides.
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| Contributor(s) |
Kloosterman TG, Hendriksen WT, Bootsma HJ, Kuipers OP, Hermans PW |
| Citation(s) |
18174343 |
| |
| Submission date |
Dec 12, 2007 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Anne de Jong |
| E-mail(s) |
[email protected]
|
| Phone |
+31 50 363 2047
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| Organization name |
university of Groningen
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| Department |
Molecular Genetics
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| Street address |
Nijenborgh 7
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| City |
Groningen |
| ZIP/Postal code |
9747 AG |
| Country |
Netherlands |
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| Platforms (1) |
| GPL6268 |
Streptococcus pneumoniae, multi strain 7k version7 (full layout) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA103825 |
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